|Three spindles (green) due to the maternal contribution and that of 2 sperm... polyspermy|
And then it was time to cut more. This time we zoomed up the evolutionary ladder to chickens. I was hesitant about chickens. The last time I 'explored' a chick embryo was in third grade. I didn't like my third grade teacher to begin with (I was vehemently opposed to wearing a sign for weeks that said ILAC - I am Lovable and Capable) and after that lesson I refused to eat eggs for 2 years. My mother thanks you. Excuse my digression. This lesson was FAR better... and proved that my third grade teacher was WRONG.
It was the heart that we went after for our chick experiment. First we had to remove the embryo from the yolk and perform the surgery - a simple nick to the region of the future heart. This nick causes the region to separate and form two twin hearts as the chick grows in culture.
|3 somite chick at initial isolation in culture and post-surgery.|
|48 hours later the chick has grown in culture and developed twin hearts. Both hearts were observed to beat.|